standardization of an enzyme-linked immunosorbent assay for detection of infectious bronchitis virus antibody.
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abstract
an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respectively. the optical densities (od) were compared with a commercial elisa kit. also, the results of elisa were compared to hemagglutination inhibition (hi) test. the correlation coefficient of the results of both elisas and hi was significant (p
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Journal title:
archives of razi institutePublisher: razi vaccine & serum research institute (rvsri)
ISSN 0365-3439
volume 59
issue 1 2016
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